• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文OA文献 >Pathogen-induced binding of the soybean zinc finger homeodomain proteins GmZF-HD1 and GmZF-HD2 to two repeats of ATTA homeodomain binding site in the calmodulin isoform 4 (GmCaM4) promoter
【2h】

Pathogen-induced binding of the soybean zinc finger homeodomain proteins GmZF-HD1 and GmZF-HD2 to two repeats of ATTA homeodomain binding site in the calmodulin isoform 4 (GmCaM4) promoter

机译：病原体诱导的大豆锌指同源域蛋白GmZF-HD1和GmZF-HD2与钙调蛋白同工型4（GmCaM4）启动子中ATTA同源域结合位点的两个重复的结合

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Calmodulin (CaM) is involved in defense responses in plants. In soybean (Glycine max), transcription of calmodulin isoform 4 (GmCaM4) is rapidly induced within 30 min after pathogen stimulation, but regulation of the GmCaM4 gene in response to pathogen is poorly understood. Here, we used the yeast one-hybrid system to isolate two cDNA clones encoding proteins that bind to a 30-nt A/T-rich sequence in the GmCaM4 promoter, a region that contains two repeats of a conserved homeodomain binding site, ATTA. The two proteins, GmZF-HD1 and GmZF-HD2, belong to the zinc finger homeodomain (ZF-HD) transcription factor family. Domain deletion analysis showed that a homeodomain motif can bind to the 30-nt GmCaM4 promoter sequence, whereas the two zinc finger domains cannot. Critically, the formation of super-shifted complexes by an anti-GmZF-HD1 antibody incubated with nuclear extracts from pathogen-treated cells suggests that the interaction between GmZF-HD1 and two homeodomain binding site repeats is regulated by pathogen stimulation. Finally, a transient expression assay with Arabidopsis protoplasts confirmed that GmZF-HD1 can activate the expression of GmCaM4 by specifically interacting with the two repeats. These results suggest that the GmZF-HD1 and –2 proteins function as ZF-HD transcription factors to activate GmCaM4 gene expression in response to pathogen.
机译：钙调蛋白（CaM）参与植物的防御反应。在大豆（最大大豆）中，在病原体刺激后30分钟内，钙调蛋白同工型4（GmCaM4）的转录被迅速诱导，但是对GmCaM4基因响应病原体的调控知之甚少。在这里，我们使用酵母单杂交系统分离了两个cDNA克隆，这些克隆编码的蛋白质与GmCaM4启动子中富含30个核苷酸的A / T序列结合，该区域包含一个保守的同源域结合位点ATTA的两个重复序列。这两个蛋白GmZF-HD1和GmZF-HD2属于锌指同源域（ZF-HD）转录因子家族。域删除分析表明，同源域基序可以与30-nt GmCaM4启动子序列结合，而两个锌指结构域则不能。至关重要的是，抗GmZF-HD1抗体与病原体处理过的细胞的核提取物孵育后形成的超移位复合物表明，GmZF-HD1与两个同源域结合位点重复之间的相互作用受病原体刺激的调节。最后，用拟南芥原生质体进行的瞬时表达测定证实，GmZF-HD1可通过与两个重复序列特异性相互作用来激活GmCaM4的表达。这些结果表明，GmZF-HD1和–2蛋白作为ZF-HD转录因子，可响应病原体激活GmCaM4基因表达。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号